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Virulence factors in uropathogenic escherichia coli (upec) causing urinary tract infections.

Journal Name:

  • Indian Journal of Basic & Applied Medical Research

Publication Year:

  • 2013

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Abstract (2. Language): 
Introduction: Escherichia. coli is the most frequent pathogen causing urinary tract infections. This study was conducted to determine the prevalence of virulence factors and antibiotic resistance in Escherichia coli (Esch coli) strains causing complicated and uncomplicated urinary tract infection (UTI). Materials and methods: A total of 100 Esch coli isolates from patient suffering from UTI and 50 Esch coli isolates (controls) from stool samples of healthy volunteers were included. These isolates were screened for virulence factors :α- haemolysin, mannose resistance and mannose sensitive haemagglutination(MRHA:P-fimbriae, MSHA: type-1 fimbriae), cell surface hydrophobicity , and serum resistance by recommended methods. Results: When 100 Esch. coli isolates from patients and 50 isolates from control (stool samples) were compared for presence of virulence factors it was seen that there was significant difference between cases and controls for P-fimbriae showing MRHA (36% vs 8%) and a-hemolysin( 54% vs 40%) (P = 0.0002, P=0.001 respectively). 52% isolates of Esch. coli from the cases had three or more virulence factors as against just 2% isolates of Esch.coli from the control. Conclusion: Multiple virulence factors were more frequent in isolates from patients with UTI as compared to controls. Isolates from uncomplicated and upper UTI were more virulent than those from complicated and lower UTI.
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