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Edilen Lactobacillus plantarum Suşlarının İzolasyonu ve Tanımlanması

Isolation of Lactobacillus plantarum from Naturally Pickles and Their Isolation and Identification

Journal Name:

  • Kahramanmaraş Sütçü İmam Üniversitesi Doğa Bilimleri Dergisi

Publication Year:

  • 2012

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
Lactobacillus plantarum species isolated from naturally prepared pickles samples. Isolates were identified using both chemical and molecular methods. Chemical identification was made by gram staining, colony morphology and catalase test. Molecular identification was made by Polymerase Chain Reaction method using different Lactic acid bacteria for specialized primers. For the rec A gene region of L. plantarum 318 bp fragments were amplified. Twenty-two L. plantarum isolates were obtained at the end of the isolation and identification. Isolated samples are stored in order to determine starter culture possibilities and genetic contents.
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Abstract (Original Language): 
Bu çalışmada doğal olarak hazırlanmış turşu örneklerinden izole edilen Lactobacillus plantarum izolatlarının kimyasal ve moleküler yöntemlerle tanımlamaları yapılmıştır. Kimyasal tanımlama gram boyama, koloni morfolojisi ve katalaz testi ile belirlenmiştir. İzole edilen laktik asit bakterilerinin moleküler tanımlaması için recA gen bölgesine spesifik primerler kullanılarak polimeraz zincir reaksiyonu (PZR) ile gerçekleştirilmiştir. recA primerleri ile gerçekleştirilen PZR işlemi sonucunda L. plantarum izolatlarından 318 bç uzunluğunda ürün elde edilmiştir. İzolasyon ve tanımlama sonucunda örneklerden 22 adet L. plantarum izolatı tespit edilmiş ve izolatlar potansiyel başlangıç kültürü kullanım imkanlarının araştırılması ve genetik içeriklerinin belirlenmesi amacıyla stoklanmıştır.
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  • Turkish

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