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MANTAR STOK KÜLTÜRLERİNİN ÜÇ FARKLI YÖNTEMLE SAKLANMASININ KARŞILAŞTIRILMASI

Journal Name:

  • Cerrahpaşa Tıp Dergisi

Publication Year:

  • 2000

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
The storage and maintenance of stock-culture collection of fungi serves to research and educational fonctions of specialized mycology laboratories. Several methods have been proposed for maintaining culture collections of fungi. In this study a general collection of stock cultures consisting of several hyaline, dematiaceous molds, dermatophytes and dimorphic primary pathogens and yeasts and yeast-like fungi has been stored at room temperatures and in a refrigerator and in a deep freezer for three years by frequent subculturing. Results.- 170 of 216 strains maintained at +4oC, 18 of 212 strains maintained at +20oC and 105 of 154 strains kept in -20oC survived the entire three year period. Both of the two methods maintain the original characters of the strains and require approximately equal time, equipment, material and space. The room temperatures storage method has been eliminated due to the risk of mit contamination. Conclusion.- Keeping stock cultures at +4oC appears to be the most convenient method for our laboratory conditions and also the composition of our actual collection.
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Abstract (Original Language): 
Çeşitli araştırmalar sırasında ayırarak tanımladığımız hava ve toprak kaynaklı 37 cinse dahil 230'un üzerinde farklı tür fırsatçı küf mantarı ile ayrıca çeşitli doğal ve klinik örneklerden ayrılmış olup laboratuvarımızda tanımlanan 208 maya ve 37 dermatofit kökeni 1996-1999 yılları arasında canlı olarak saklanmaktadır. Koleksiyonumuzun yapısına ve laboratuvarımız koşullarına uygun saklama yönteminin belirlenebilmesi amacıyla bir çalışma planlanmış ve yürütülmüş olup üç yıllık sonuçları sunulmaktadır. Bu çalışmada toplam 216 köken deneye alınmış üç ayrı saklama yöntemi uygulanmıştır. Üç yıl sonunda +4oC'de saklanan 216 kökenin 170'i, +20oC'de saklanan 212 kökenin 18'i ve -20oC'de saklanan 154 kökenin 105'i amaca uygun şekilde korunmuştur. Bizim laboratuvar koşullarımıza ve koleksiyonumuzun yapısına en uygun +4oC'de saklama yöntemi olduğu görülmüştür.
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REFERENCES

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