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Chlorpyrifos ethyl' in rat pankreası üzerine etkisi

The effects of chlorpyrıfos-ethyl on pancreas in rats

Journal Name:

  • Süleyman Demirel Üniversitesi Tıp Fakültesi Dergisi

Publication Year:

  • 2005

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
Pancrease is an organ whic h realtively vulnareble to various xenobiotics. It is reported that organophospahates cause acute pancreatitis at approximately 12% rate. We aimed to investigate effects of chlorpyrifos-ethyl (CE) [O,O-diethyl-O-(3,5,6-trichloro-2-pyridyl)phosphorothioate], an organophosphate insecticide, on pancrease at the maximum tolerable dose within various duration. Study groups designed as control group, CE treated (administered single tolerable dose) adult group and CE treated (administered single tolerable dose) infantil group. Each group divided into three subgroups. Animals were sacrificied at days 2., 7. and 14. We examined effects of CE on serum activities of cholinesterase (ChE), amylase and lipase enzymes in plasma and, blood levels of malondialdehyde (MDA) in erythrocytes. Compared to control group, ChE activity was found to be significantly decreased while amylase activity and MDA level were found to be sigificantly elevated in CE treated group. Compared to CE treated group, lipase activity was found to be increased in CE treated group. Based on the findings that were obtained from this study we can conclude that maximum tolerable dose of CE may cause pancreas disorder that is probably not directly related to increased oxidative stress or inhibition of cholinesterase enzyme activity.
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Abstract (Original Language): 
Pankreas bazı ksenobiotiklere göreceli olarak daha hassas bir organdır. Organofosfat zehirlenmelerinde ortalama % 12 oranında akut pankreatite rastlandığı bildirilmiştir. Bu çalışmada organofosfat insektisitlerden chlorpyrifos ethyl (CE)'in tolere edilebilir maksimum dozda pankreas üzerine olası etkilerini genç ve erişkin ratlarda araştırmayı planladık. Çalışma grupları; genç kontrol grubu, genç CE verilen grup ve erişkin CE verilen grup şeklinde düzenlendi. Bu gruplarda üçer alt gruba ayrılarak deney başladıktan sonra 2, 7 ve 14 gün sonra kan örnekleri alındı. Her grubun deney süresinin bittiği günde kan örnekleri alınarak plazmada kolinesteraz (ChE), amilaz ve lipaz aktiviteleri ile malondialdehit (MDA) düzeyleri ölçüldü. Kontrol grubuyla karşılaştırıldığında CE uygulanan grubun ChE aktivitesinin anlamlı olarak azaldığı görüldü. CE uygulanan grup ile kontrol grubu karşılaştırıldığında amilaz aktivitesinin ve MDA düzeyinin anlamlı olarak arttığı görüldü. Sonuç olarak bu bulgulardan CE'nin maksimum tolare edilebilir dozda ratlara uygulandığında hafif pankreas hasarına neden olabileceği fakat bunun oksidatif stres artışı veya kolinesteraz enzim aktivitesin inhibisyonu ile doğrudan yakın ilişki halinde olmadığı düşünüldü.
FULL TEXT (PDF): 
PDF icon arastirmax-chlorpyrifos-ethyl-rat-pankreasi-uzerine-etkisi.pdf
  • 4
19-22
  • Turkish

REFERENCES

References: 

1. Hsiao CT, Yang CC, Deng JF, Bullard MJ, Liaw SJ. Acute pancreatitis following orgaphosphate intoxication. J Toxicol Clin Toxicol. 1996;34:343-47.
2. Sahin I, Onbasi K, Sahin H, Karakaya C, Ustun Y, Noyan T. The prevelance ofpancreatitis in organophosphate poisonings. Hum Exp Toxicol. 2002;21:175-77.
3. Hegyi P, Takacs T, Tiszlavicz L, Czako L, Lonovics J. Recovery ofexocrine pancreas six months following pancreatitis induction with L-arginine in streptozotocin-diabetic rats. J Physiol Paris. 2000;94(1):51-5.
4. A.J. Bone, D.J. Gwillam, Animal models ofinsulin-dependent diabetes mellitus. 1998, Chapter 16, pp.
16.1-16.
5. The Expert Committee on The Diagnosis and Classification ofDiabetes Mellitus. Report ofthe expert committee on the diagnosis and classification ofdiabetes mellitus. Diab Car 1999;22 (Supplement 1): S5-S19.
6. Rizos E, Liboropoulos, Kosta P, Efremidis S, Elisaf S. Carbofuran-induced acute pancreatitis. JOP. J Pancreas 2004; 5(1):44-7.
7.
Gökal
p O, Mollaoğlu H, Yılmaz HR, Altuntaş İ. Fenthion'un pankreas üzerine etkileri: Vitamin E ve C'nin rolü. Süleyman Demirel Üniversitesi Tıp Fakültesi Dergisi 2003; 10(2): 21-3.
8.
Mollaoğl
u H, Yılmaz HR, Gökalp O, Altuntaş İ. Methidathion'un pankreas üzerine etkileri: Vitamin E ve C'nin rolü. Van Tıp Dergisi 2003; 10: 98-100.
9. Tinoco R, Halperin D. Poverty, production, and health: inhibition oferythrocyte cholinesterase via occupational exposure to organo-phosphate insecticides in Chiapas, Mexico. Arch Environ Health 1998; 53: 29-35.
10. Gokalp O, Buyukvanlı B, Cicek E, Ozer MK, Koyu A, Altuntas I, Koylu H. The effects ofdiazinon on pancreatic damage and ameliorating role ofvitamin E and vitamin C. Pes Bio and Phy2005; 81(2): 123-8.
11.
Demiri
n H, Büyükvanlı B, Öztürk Ö, Gökalp O, Yılmaz HR, Koyu A, Altuntaş İ, Köylü H. Karaciğerde organofosfat insektisit fosalonun yaptığı hasar: vitamin E ve C'nin etkileri. 30. Ulusal Fizyolojik Bilimler Kongresi. 31 Ağustos-3 Eylül 2004, Konya-TURKEY
12. Dabrowski S, Hanke W, Polanska K, Makowiec-Dabrowska T, Sobala W. Pesticide exposure and birthweight: an epidemiological study in Central Poland. Int J Occup Med Environ Health 2003;16:31-9.
13. Lotti M. Promotion oforganophosphate induced delayed polyneuropathy by certain esterase inhibitors. Toxicology 2002;27:181-182:245-248.Carr RL, Richardson JR, Guarisco JA, Kachroo A, Chambers JE, Couch TA, Durunna GC, and Meek EC. Effects of PBC exposure on the toxic impact oforganophosphors insecticides. Toxicol Sci 2002;67:311-21.
14. Karalliadde L. Organophosphorus poisoning and
anesthesia. Anesthesia 1999;54:1073-88.
15. Altuntaş İ, Delibaş N. The effects ofFenthion on lipid peroxidation and some liver enzymes: The possible protective role Vitamins E and C. Turk J Med Sci.
2002;32:293-97.
16. Gültekin F, Delibaş N, Yaşar S, Kılınç İ. In vivo changes in antioxidant systems and protective role ofmelatonin and a combination of vitamin C and vitamin E on oxidative damage in erythrocytes induced by chlorpyrifos-ethyl in rats. Arch Toxicol 2001;75:88-
96.
17. Bagchi D, Bagchi M, Hassoun EA, Stohs SJ. In vitro and in vivo generation ofreactive oxygen species. DNA damage and lactate dehydrogenase leakage by selectedpesticides. Toxicol 1995;104:129-40.
18. Appenroth D, Frög S, Kersten L, Splinter FK. Protective effects ofvitamin E and C on Cisplatin nephrotoxicity in developing rats. Arch Toxicol1997;71:677-83.
19. Dressel TD, Goodale RL Jr, Zweber B, Borner JW. The effect of atropine and duct decompression on the evolution ofDiazinon-induced acute canine pancreatitis. AnnSurg. 1982Apr;195(4):424-34.
20. Liu S, Oguchi Y, Borner JW, Runge W, Dressel TD, Goodale RL. Increased canine pancreatic acinar cell damage after organophosphate and acetylcholine or cholecystokinin. Pancreas 1990;5(2):177-82.
21. Panieri E, Krige JE, Bornman PC, Linton DM. Severe necrotizing pancreatitis caused by organophosphate poisoning. JClinGastroenterol. 1997;25:463-65.

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