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Direkt pulpa kaplama çalışmasında rat modeli

Rat as a model for direct pulp capping research

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Abstract (2. Language): 
The aim of this review is to evaluate the suitability of rat teeth in preclinical evaluation of medical materials for direct pulp capping. The international standards state clearly that only non–rodent mammals are suitable species for animal research in dentistry. However, in the past half of the century numerous research have been published using rat teeth in order to evaluate direct pulp capping, pulpotomies and tissue reactions after pulp exposure. Several studies showed that the healing of rat pulp tissue after direct pulp capping was histologically comparable with human’s and other animal species’ pulp tissue. In the rat teeth including pulp tissue, basic biological reactions of the pulp tissue and the interactions seen during the different stages of wound healing are comparable with those of the other mammal species. Rat teeth are a valid study model in order to provide valuable data concerning pulp tissue reaction after direct pulp capping in dentistry. Therefore, the use of rats may significantly reduce the number of currently used higher animals in research. Tests in higher developed animals should be limited to experiments which clarify inconsistent results. However, some technical difficulties, like the small size of rat teeth must be dealt with before undertaking any research.
Abstract (Original Language): 
Bu derlemenin amacı direkt pulpa kaplaması için medikal materyallerin klinik öncesi değerlendirilmesinde rat dişlerinin uygunluğunu değerlendirmektir. Uluslararası standartlara göre, diş hekimliğinde hayvan çalışmaları için yalnızca kemirgen olmayan memelileri türlerinin direkt pulpa kaplamasında amacıyla kullanım uygunluğu oldukça açıktır. Öte yandan geçmiş yarım yüzyılda birçok çalışma, rat dişleri üzerinde pulpanın ağız ortamına açılmasından sonraki direkt pulpa kaplamasını, pulpotomileri ve doku reaksiyonlarını değerlendirmiştir. Birçok çalışmada rat pulpa dokusunun direkt pulpa kaplamasından sonraki iyileşmesinin histolojik olarak insan dişlerinin ve diğer hayvan türlerinin pulpa dokusu ile karşılaştırılabilir olduğu gösterilmiştir. Pulpa dokusu içeren rat dişlerinde, pulpa dokusunun temel biyolojik tepkimeleri ve rat dişlerinin yara iyileşmesinin farklı aşamaları boyunca görülen etkileşimler, diğer memeli türleri ile karşılaştırılabilir. Diş hekimliğinde rat dişleri, direkt pulp kaplaması sonrasında pulpa dokusu tepkimelerini içeren kayda değer veriler elde etmede geçerli bir çalışma modelidir. Bundan dolayı ratların kullanılması evrim düzeyi yüksek memelilerin kullanıldığı çalışmaların sayısını önemli ölçüde azaltabilmektedir. Sonuçları belirgin olmayan çalışmalarda evrim düzeyi yüksek hayvanların kullanımı sınırlandırılmalıdır. Her ne kadar küçük diş yapısına sahip olmaları gibi bazı teknik zorluklara sahip olsalar da, rat dişleri ile yapılan herhangi bir çalışmanın üstesinden gelinebilir
FULL TEXT (PDF): 
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