DEVELOPMENT AND VALIDATION OF PALIPERIDONE IN BULK AND PHARMACEUTICAL FORMULATION BY UV SPECTROSCOPY

A simple, precise and economical Zero-order Spectroscopy (Method A), Area Under Curve [AUC] (Method B) UV-Spectrophotometric methods have been developed and validated for the estimation of Paliperidone in bulk and its formulation. The solutions of standard and sample were prepared using 0.1N HCl. Paliperidone was estimated at 238 nm for the zero order and, AUC of Paliperidone was measured in between 232-244 nm. Beer's law was obeyed in the concentration range of 3-18 ug / ml with r2 value 0.9998, 0.999 for Zero order method and AUC spectroscopy. The precision expressed as relative standard deviation, which was within 2.0 % for the above three methods. The proposed methods were successfully applied for the determination of Paliperidone in bulk and pharmaceutical formulations. In addition, the proposed methods are simple, easy to apply, low-cost, and requires relatively inexpensive instruments.