You are here

Home » Content

OPTIMIZATION AND CHARACTERIZATION OF BIOSURFACTANT PRODUCING MICROBES ISOLATED FROM OIL CONTAMINATED SOIL AND EXPRESSION OF BIOSURFACTANT GENES IN E.COLI

Journal Name:

  • The International Journal of Pharmaceutical Research and Bio-Science

Publication Year:

  • 2013

Key Words:

Author Name
Abstract (2. Language): 
The present study is carried out by optimization, isolation and characterization of biosurfactant producing microbes and also expression oil degrading genes from oil degrading microbes to non-oil degrading microbes. Bioremediation is a process in which the environment can cleanup through microbes. The various types of soil sample were collected from different regions of Unnao and Lucknow. The soil was contaminated with oil. Total 15 cultures were isolated through serial dilution method out of which 4 cultures were characterized through Bergey's manual. These cultures were tested for oil degradation by test tube method in small scale and large scale method. Four types of oils were used: petrol, diesel, kerosene and vegetable oil. The best results were obtained for all these cultures against petrol. The identified cultures were: M.luteus, S.aureus, B.cereus, N.flavescens. Further plasmid isolation was done by alkaline denaturation method and transformation process was completed by heat shock treatment. The host cells used as E.coli which was not oil degrading microbes and after transformation the oil degrading capacity was developed which shows positive results and also optimization was done to enhance the capacity of oil degrading microbes. The optimization parameters include carbon source (glucose, dextrose, maltose, sucrose, beef extract), nitrogen source (urea, peptone, KNO3, NH4Ü), metal ion (Zn, Pb, Ca, Mg) and pH (5,6,7,8).
Bookmark/Search this post with
FULL TEXT (PDF): 
PDF icon ijprbs_283.pdf
  • 2
46-67
  • English

REFERENCES

References: 

1. Tabatabaee. A, Assadi. M M, Noohi. A A, Sajadian.V A, Isolation of Biosurfactant Producing Bacteria from Oil Reservoirs. Iranian J Env Health Sci Eng, 2005, 2(1):6-12.
2. Gilbert.P D and Higgins.I J, The Microbial Degradation of Crude Mineral Oils at Sea, Journal of General Microbiology,1978, 108:63-70.
3. Kosaric. N, Biosurfactants in industry. Pure & Appl. Chern, 1992, 64(11):1731-
1737.
4. Sarubbo, L.A, Production and Stability Studies of the Bioemulsifier Obtained from a Strain of Candida glabrata UCP 1002. J. Biotechnol, 2006, 9: 400-406.
5. T. Priya and G. Usharani, Comparative Study for Biosurfactant Production by Using
Bacillus subtilis and Pseudomonas aeruginosa. Botany Research International, 2009, 2 (4): 284-287.
6. Rodrigues, L.R., J.A. Teixeira, H.C. Mei and R, Oliveira Physicochemical and Functional Characterization of a Biosurfactant Produced by Lactococcuslactis 53, Colloids and Surfaces B, Biointerfaces,
2006, 49: 79-86.
7. Aneja, K.R., Experiments in Microbiology, Plant Pathology and Biotechnology. New Age International (P) Limited: New Delhi,
2003, Vol. 4. P. 607.
8. Erem F, Certel. M, Karakas. B, Identification of Bacillus species isolated from ropey breads both with classical methods and API identification
Available Online At www.ijprbs.com
Research Article
Amit Pandey, IJPRBS,
2013; Volume 2(2): 46-67
ISSN: 2277-8713
IJPRBS
kits.Akdenizüniversitesiziraatfakültesidergisi , 2009, 22(2), 201-210.
9. K. Santhini, J. Myla, S. Sajani and G.Usharani, Screening of Micrococcus Sp from Oil Contaminated Soil with Reference to Bioremediation, Botany Research International, 2 (4): 248-252.
Available

Thank you for copying data from http://www.arastirmax.com