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UV SPECTROPHOTOMETRIC METHOD FOR IDENTIFICATION AND ESTIMATION OF CLONAZEPAM IN TABLET DOSAGE FORM

Journal Name:

  • The International Journal of Pharmaceutical Research and Bio-Science

Publication Year:

  • 2012

Key Words:

Author NameUniversity of Author
Abstract (2. Language): 
The present research work describes the spectrophotometric method for the identification and estimation of Clonazepam in bulk and pharmaceutical dosage form. Identification test is proposed based on distinctive UV spectra of Clonazepam under acidic and alkaline condition. The drug exhibited UV absorpti on maximum at 271 nm in 0.01 N HCl which underwent Hyper chromic and Blue shift to 263 nm when sample was heated in boiling water bath for 2 hours. The drug exhibited UV absorption maximum at 364 nm in 0.01 N NaOH which underwent Hypo chromic and Red shift to 387 nm when sample was heated in boili ng water bath for 2 hours. For the quantitative estimation, the stock solution was prepared in methanol and the drug was determined at absorption maximum of 309 nm. Beers law was obeyed in the concentration range of 4-28 |ig/ml having regression line equation y = 0.0377x + 0.0005 with correlation coefficient of 0.9998. Results of the analysis were validated statistically and recovery was found between 98.81-1 01.03%. The method can be used for routine quality control analysis of Clonazepam formulation
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  • English

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