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GENETIC VARIATION ON HIBISCUS SPECIES BY USING RAPD MARKERS

Journal Name:

  • Advance Research in Pharmaceuticals and Biologicals

Publication Year:

  • 2012

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Abstract (2. Language): 
Hibiscus rosa-sinensis is a attractive ornamental plant with brilliant red, double whorled crumpled red, orange, pink, double whorled crumpled pink and white flowers. This study was undertaken for genetic analysis within the four varieties of Hibiscus rosa-sinensis by using RAPD markers. The method involves a extraction of DNA from leaves of Hibiscus rosa-sinensis and yield of DNA ranged from 158- 200μg and purity (ratio) was between 1.3-1.6 indicating minimum level of contaminating metabolites and the isolated DNA was used for RAPD analysis. Genetic analysis was made by using four primers of RP1, RP2, RP3 and RP4. RAPD results produced scorable banding pattern with different primer used in the experiment. From a total of 6 samples distinct DNA fragments ranging from 0.3 to 2.5 kb were amplified by using selected random decamer primers. On this basis genetic similarity was evaluated on the absence or presence of bands. The genetic distance was very close within the varieties and also among the species. Thus, these RAPD markers have the potential for the identification of species/varieties and characterization of genetic variation within the varieties of species. This is also useful in Hibiscus breeding programs and provides a major input into conservation biology.
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