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TAMOKSIFEN VERİLEN SIÇANLARDA KARACİĞER POLİAMİN DÜZEYLERİNİN HPLC İLE İNCELENMESİ

Journal Name:

  • İstanbul Tıp Fakültesi Dergisi

Publication Year:

  • 2000

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
Tamoxifen and tiver polyamine tevels in rats. The observation in humans and in experimen-tal animals suggests dıat the anti-estragenic drug tanıoxİfen nıay be associated with Üver can-cer incidence. Poiyamines, which are present in both prokaryotic and eukaryotic ceîls havc bc-en shown to stİmulatc angiogenesis and theİr concentrations have been found to be related to the proliferation and vaıious types of neoplasms. In this sludy, it was aimed to examine the polyamine levels in liver tissue homogenates following tamoxİfen administration to rats. Female Wistar albino rats were given tamoxifcn citrate intraperitoneally for 26 days in a dose of either 10 mg/kg or 20 mg/kg body weight. At the end of experimental period, animals were sacrified and livers were excised and prepared for polyamine determinations (putrescine, spermidine and spermine) by high performance liquid ehromalography (HPLC). Histological evaluations of the tissues were also carried out. Our dala revealed that polyamine concentrations in liver tissue were not affected by tamoxi-fen. Minör histological changes were observed fo31owing Periodic Acid Schİff dye.
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Abstract (Original Language): 
İnsan ve deney hayvanlarında yapılan çalışmalar sonucunda atıtiöstrojenİk bir ajan olan ta-moksifenin, karaciğer kanseri oluşumunda roî oynadığı düşünülmektedir. Prokaryot ve ökar-yot hücrelerin önemli bir bileşeni olan poliaminlerin konsantrasyonu, hücre proliferasyonu ve diferansiyasyonunun yüksek olduğu ve damarlanmanm arttığı hallerde artmış bulunmuştur. Ayrıca tümör oluşumu üe poliamin konsantrasyonu arasında da bir ilişki olduğu belirlenmiştir. Bu çalışmada, tamoksifen uygulanan sıçanlarda karaciğer dokusu poliamin düzeylerinin incelenmesi amaçlanmıştır. Wistar albino dişi sıçanlara 26 gün süreyle 10 mg/kg vc 20 m«/kg dozlarda tamoksifen silrat intrapcrİtoneal yol ile uygulanmış, deney süresi sonunda hayvanlar sak-rifiye edilerek HPLC yöntemi ile poliamin (putresin, speımidiıı ve spermin) tayini için karaciğer doku homojenatları kullanılmıştır. Karaciğer dokusunun genel yapısal özelliklerindeki herhangi bir değişimi gözlemlemek amacıyla hazırlanan preparatlarda histolojik İnceleme yapılmıştır. Çalışma sonunda, tamoksifen uygulamasının karaciğer dokusu poliamin düzeylerini etkilemediği, bununla beraber minör histolojik değişiklikler meydana getirdiği görülmüştür
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  • 1
13-18
  • Turkish

REFERENCES

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