OCTREOTIDE'NİN YENİDOĞAN STREPTOZOTOCIN DİYABETİK SIÇAN BÖBREĞİ VE IGF-1 DÜZEYLERİ ÜZERİNE ETKİLERİ

Makale İçerik Bilgileri
Makale Dili: 
Türkçe
Anahtar Kelimeler: 
Yeni doğan STZ-diyabeti
Renal IGF-1
Octreotide
Türkçe Özet: 

Somatostatin ve analoglarının (Octreotide-
SMS 201-995) diyabet ve komplikasyonlarının
tedavisinde yararlı etkiye sahip olduğu çeşitli
çalışmalarla bildirilmiştir. Çalışmamızda yenidoğan
(YD) Wistar sıçanlardan oluşan 4 grup
kullanldı. Doğumun 2. günü (YD:2) 1. ve 2.
grup YD-STZ-diyabetik grup (100 mg/kg, tek
doz, i.p., doğumun 2. günü), 2.grup; Octreotide
tedavili YD-STZ-diyabetik grup (200 µg/kg/
gün, i.p., 12.haftadan itibaren 6 hafta), 3.grup;
Octreotide uygulanan diyabetik olmayan grup
(200µg/kg/gün, i.p., 12.haftadan itibaren 6 hafta),
4.grup; sağlıklı kontrol olarak ayrıldı. STZ
uygulanan gruplarda 12.haftaya kadar sağlıklı
kontrole göre kan glukozunda belirgin artış
gözlenirken, octreotide uygulanan diyabetik
grupta kan glukoz düzeyi düştü. YD-STZdiyabetik
grupta böbrek ağırlıklarında sağlıklı
kontrol grubuna kıyasla artış saptandı. Octreotide
uygulanan diyabetik grupda ise diyabetik
gruba kıyasla böbrek ağırlıkları azaldı. STZdiyabetik
gruplarda serum IGF-1 düzeylerinin
octreotide uygulanan diyabetik grupta düştüğü
saptandı. Glomerül boyutlarının YD-STZ-diyabetiklerde
sağlıklı kontrole kıyasla arttığı gözlendi.
Diyabetik gruplarda bazal membran kal
ınlaşması, mezangial matriks ve mezangial
hücre artışı gözlendi. Diyabetik grupta glomer
üllerde kuvvetli IGF-1 immunpozitifliği gözlendi.
Tedavili YD-STZ-diyabetik grupta, diyabetik
ve sağlıklı kontrol gruplarına oranla
glomerüllerde ve tubüllerde oldukça zayıf IGF-
1 immunpozitifliği, sağlıklı kontrol grubunda
tubüllerde IGF-1 pozitif immunreaksiyonu saptand
ı. Mikroalbüminüri düzeyi diyabetik gruba
kıyasla tedavili diyabetik grupta azaldı.
Sonuç olarak; Octreotide uygulamasının
YD-STZ-diyabet modelinde diyabetik nefropatinin
ilerlemesini yavaşlattığı ve renal doku hasar
ını düzelttiği tespit edildi.

Key Words: 
Neonatal STZ-diabetes
Renal IGF-1
Octreotide
İngilizce Özet: 

It has been shown by the studies that somatostatin and its analogues (octreotide)
have a useful effect on the treatment of diabetes and its complications. In this study 4 groups of newborn Wistar
rats were prepared. 1st and 2nd Group; Neonatal STZ (n-STZ)-diabetic group (100 mg/kg, single dose, i.p. on the
second day of birth). 2nd Group; Octreotide treated n-STZ-diabetic group ( 200 µg/kg/day, i.p, from 12.week to
18.week ). 3rd Group; Octreotide treated non diabetic group ( 200 µg/kg/day,i.p., from 12.week to 18.week).
4.Group; Control group. In all STZ treated groups (1st ,2nd group) while a significant increase in the blood glucose
compared to healty controls until 12th week.
Results.- A decrease in the blood glucose levels of octreotide treated diabetic group have been observed. Kidney
weights were increased in STZ diabetic groups compared to healty control groups. A significant increase in
octreotide treated diabetic groups was determined. Serum IGF-1 levels were found decreased in octreotide
treated diabetic group. Glomerule sizes increased in STZ diabetic groups compared to controls. There were basal
membrane thickening and significant increase in mesangial matrix and mesangial cells in the diabetic groups.
Renal tissue IGF-1 immunoreactivity on the kidney tubules of the control group was found higher than the diabetic
group. IGF-1 immunosreactivity was generally seen on the glomerules of the STZ-diabetic group. In Octreotide
treated nSTZ-diabetic group weaker IGF-1 immunreaction observed at glomerules and tubules compared to
nSTZ-diabetic and healthy control group. The microalbuminuria levels of the treated diabetic group were found
reduced when compared with the diabetic group.
Conclusion.- We observed that the dose of octreotide and the period has an important contribution for
preventation and improvement of nephropathy and prevent the renal tissue damage. on the newborn STZ-diabetic
rats.

Yazar Bilgileri
1. Yazar
Yazar Adı: 
Matem Tunçdemir
Yazar Üniversitesi: 
İstanbul Üniversitesi
Yazar Fakültesi: 
Cerrahpaşa Tıp Fakültesi
Yazar Anabilim Dalı: 
Tıbbi Biyoloji
2. Yazar
Yazar Adı: 
Melek Öztürk
Yazar Ünvanı: 
Profesör
Yazar Üniversitesi: 
İstanbul Üniversitesi
Yazar Fakültesi: 
Cerrahpaşa Tıp Fakültesi
Yazar Anabilim Dalı: 
Tıbbi Biyoloji
Makale Künye Bilgisi
Makalenin Yayımlandığı Dergi: 
Cerrahpaşa Tıp Dergisi
Makale Yayın Yılı: 
2002
Cilt/Sayı: 
33
Sayı: 
2
Sayfa Aralığı: 
117-123
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