ALKOLİK YAPILAN GEBE SIÇANLAR VE YAVRULARINDA NK AKTİVASYONU İLE IL-2, IFN-γ ve CD19 ETKİLEŞİMİ

Makale İçerik Bilgileri
Makale Dili: 
Türkçe
Anahtar Kelimeler: 
Gebelik
Etanol
CD19
İmmunite
NK
IL-2
Yavrular
Türkçe Özet: 

Çalışmamız NK hücrelerinin, alkolik
yapılan gebe sıçanlar ve yavrularında IL-
2 , IFN-γ ve CD 19 ile nasıl etkileşim içinde
olduğunu göstermek amacıyla planland
ı. Toplam 80 Wistar albino soyu dişi sı-
çanda yapılan araştırmanın grupları; 1)
Kontrol Grubu (K) (n=10) 2) Gavajla etanol
uygulanan grup (G) (n=10) 3) Kontrol
gebe grubu (KG) (n=10) 4) Gavajla etanol
uygulanan gebe grubu (GG) (n=10) 5)
Kontrol 10 günlük yavru (KY10) (n=10) 6)
Kontrol 30 günlük yavru (KY30 ) (n=10) 7)
Gavajla etanol uygulanan gebenin 10 günl
ük yavrusu (GY10) (n=10) 8) Gavajla etanol
uygulanan gebenin 30 günlük yavrusu
(GY30) (n=10) şeklinde belirlendi.
NK değerlerinin [(K: %54.90 ± 10.86 ile
G: % 38.40 ± 3.43), (KG: %40.00±2.1 ile
GG:%37.1±2.10 )] grupları arasında, 10 ve
30 günlük yavru [(KY10: % 30.2± 2.1 ile
GY10: %13.0 ± 0.7), (KY30: %32.28 ± 2.6
ile GY 30: % 20.75 ± 1.2)] grupları arasında
anlamlı düşüş olduğu belirlendi. IL-2
değerlerinin [(K: 86.5 ± 1.3 pg/ml ile
G:71.0±2.4 pg/ml.) (KG: 65.9 ± 1.1 pg/ml
ile G.G: 60.9 ±2.1 pg/ml.)] grupları arasında,
10 ve 30 günlük yavru [( KY10: 75.4 ±
3.2 pg/ml ile GY10: 63.0 ± 3.2 pg/ml ),
(KY30: 76.0 ± 3.4 pg/ml ile GY30: 70.0 ±
2.6 pg/ml)] grupları arasında anlamlı dü-
şüş olduğu görüldü. IFN-γ değerlerinin
[(K:1250 ± 29.6 pg/ml ile G: 860 ± 27.3
pg/ml ), (KG: 720 ± 13.6 pg/ml ile GG: 570
± 9.1 pg/ml )] grupları arasında, 10 ve 30
günlük yavru [(KY10: 850 ± 25.0 pg/ml ile
GY10: 520 ± 17pg/ml ) (KY30: 900 ± 10
pg/ml ile GY30: 640 ± 16.10 pg/ml )] gruplar
ı arasında anlamlı azalma gözlendi.
CD19 değerleri [(K: %25.73 ± 3.07 ile G:
%23.83 ± 1.6) grupları arasında anlamlılık
belirlenemedi. (KG: %23.98 ± 1.7 ile GG:
%18.46 ± 1.7) grupları arasında anlamlı
düşüş görüldü. 10 ve 30 günlük yavru
[(KY10: %11.15 ± 1.7 ile GY10: %32.5 ±
2.0 ) (KY30: %21.35 ± 1.2 ile GY30: %30.8
± 1.8)] grupları arasında anlamlı artış belirlendi.
Gavaj yöntemiyle alkol uygulanan dişi
sıçanlarda ve bunların gebelik döneminde
kontrol grubuna göre NK aktivitesinin anlaml
ı olarak azaldığı, buna paralel olarak
CD-19, IL-2 ve IFN-γ düzeyinde de azalma
olduğu görüldü. Gebelik döneminde alı-
nan alkolün, 10 günlük yavrularda NK,
IL-2, IFN-γ düzeyinde, 30 günlük yavrulara
göre daha güçlü supresif etkisi olduğu
belirlendi. Gerek 10 gerekse 30 günlük
yavrularda CD19 düzeyinde anlamlı artı-
şın olduğu gözlendi. Gebelik sürecinde annede
gelişen özel immün tolerans sistemlerinin
teratojenik faktörlerin başında gelen
alkol ile değişime uğradığı sonucuna
varıldı.

Key Words: 
Pregnancy
Ethanol
CD19
Immunity
NK
IL-2
Offspring
İngilizce Özet: 

Our study aims to show how
the NK cells interact with the parameters of
IL-2, IFN-γ and CD 19 in the alcoholic pregnant
rats and their offspring. Several experimental
animal models have been described
for the study that was carried out on a total
number of 80 Wistar albino female rats.
Design.- In our study, rats were divided into
8 groups; 1) Control group (K) (n=10) 2)
Group that received ethanol through gavage
(G) (n=10) 3) Control pregnant group (KG)
(n=10) 4) Pregnant group that received
ethanol through gavage (GG) (n=10) 5)
Control offspring (10 days old) (KY10)
(n=10) 6) Control offspring (30 days old)
(KY30) (n=10) 7) 10-day-old offspring of
pregnant rats that received ethanol through
gavage (GY10) (n=10) 8) 30 day old offspring
of pregnant rats that received ethanol
through gavage (GY30) (n=10).
Results.- Data from this study show that
there has been a considerable decrease in
the NK values the successive groups [(K: %
54.90±10.86 and G: % 38.40±3.43), (KG: %
40.00±2.1 and GG: % 37.1±2.10)] and the
groups of 10 to 30 day old offspring of rats
[(KY10: %30.2±2.1 and GY10: %13.0±0.7),
(KY30: %32.28±2.6 and GY30: %20.75±
1.2)] respectively. A significant decrease is
observed in the IL-2 values between the
consecutive groups [(K: 86.5±1.3 pg/ml and
G: 71.0±2.4 pg/ml), (KG: 65.9±1.1 pg/ml and
GG: 60.9±2.1 pg/ml )] and the groups of 10
to 30 day old offspring of rats [(KY10:
75.4±3.2 pg/ml and GY10: 63.0±3.2 pg/ml),
(KY30: 76.0±3.4 pg/ml and GY30: 70.0±2.6
pg/ml)] respectively.
The IFN-γ values indicated a considerable
decrease among the successive groups [(K:
1250±29.6 pg/ml and G: 860±27.3 pg/ml)
(KG: 720±13.6 pg/ml and GG: 570±9.1 pg/
ml)] and the groups of 10 to 30 day old offspring
of rats [(KY10: 850±25.0 pg/ml and
GY10: 520±17 pg/ml) (KY30: 900±10 pg/ml
and GY30: 640±16.10 pg/ml) ] respectively.
No significant changes were observed in the
CD-19 values between the groups [(K: %
25.73±3.07 and G: % 23.83±1.6) while there
was a significant decrease between the
groups (KG: %23.98±1.7 and GG: % 18,46±
1.7)] The CD-19 values displayed a considerable
increase among the consecutive
groups of [(KY10: %11.15±1.7 and GY10: %
32.5±2.0) (KY30: %21.35±1.2 and GY30: %
30.8±1.8)] respectively.
Conclusions.- It has been observed
that in the female pregnant rats that received
alcohol through gavage, the NK activity
falls considerably according to the control
group and in parallel to that there is a
decrease in the levels of the parameters CD
19, IL-2 and IFN-γ. Compared to the 30-day
old-offspring, a more suppressive effect was
observed in the NK, IL-2 and IFN-γ levels of
the 10 day old offspring of the rats that received
alcohol during the period of pregnancy.
Also in the CD-19 level, a significant
increase is observed both in 10 and 30 day
old offspring. These results point out the fact
that the teratogenic factors like alcohol are
primarily responsible for the profound and
harmful effects on the special immune tolerance
systems that have developed through
the period of pregnancy of the mother.

Yazar Bilgileri
1. Yazar
Yazar Adı: 
Sibel Akyol
Yazar Üniversitesi: 
İstanbul Üniversitesi
Yazar Fakültesi: 
Cerrahpaşa Tıp Fakültesi
Yazar Anabilim Dalı: 
Fizyoloji
2. Yazar
Yazar Adı: 
Halil Tunalı
Yazar Ünvanı: 
Profesör
Yazar Üniversitesi: 
İstanbul Üniversitesi
Yazar Fakültesi: 
Cerrahpaşa Tıp Fakültesi
Yazar Anabilim Dalı: 
Fizyoloji
3. Yazar
Yazar Adı: 
Bayram Kıran
Yazar Üniversitesi: 
İstanbul Üniversitesi
Yazar Fakültesi: 
Çapa Tıp Fakültesi
Yazar Anabilim Dalı: 
İmmünoloji
4. Yazar
Yazar Adı: 
Özdemir İlter
Yazar Ünvanı: 
Profesör
Yazar Üniversitesi: 
İstanbul Üniversitesi
Yazar Fakültesi: 
Cerrahpaşa Tıp Fakültesi
Yazar Anabilim Dalı: 
Çocuk Sağlığı ve Hastalıkları
Makale Künye Bilgisi
Makalenin Yayımlandığı Dergi: 
Cerrahpaşa Tıp Dergisi
Makale Yayın Yılı: 
2001
Cilt/Sayı: 
32
Sayı: 
1
Sayfa Aralığı: 
43-50
PDF Dosyası: 
application/pdf iconarastirmax_1604_pp_43-50.pdf
Makalenin Yayınlandığı Web Sitesindeki Linki: 
Makaleyi Dergi Web Sitesinden İndirin
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